Skip Navigation

Bioscience Horizons 2008 1(1):28-37; doi:10.1093/biohorizons/hzn008
This Article
Right arrow Full Text Freely available
Right arrow FREE Full Text (PDF) Freely available
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Google Scholar
Right arrow Articles by Goldsworthy, M. J. H.
PubMed
Right arrow Articles by Goldsworthy, M. J. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

© Oxford University Press 2008

Gene expression of Pseudomonas aeruginosa and MRSA within a catheter-associated urinary tract infection biofilm model

Michael John Howard Goldsworthy*

School of Biosciences, Exeter University, Geoffrey Pope Building, Stocker Road, Exeter, EX4 4QD, UK

* Corresponding author: 6 Woodbine Terrace, Exeter, Devon, EX4 4LJ, UK. Tel: +44 01392 269170. Email: m.j.h.goldsworthy{at}ex.ac.uk

Supervisors: Sara Burton and Hilary Lappin-Scott, School of Biosciences, Exeter University, Geoffrey Pope Building, Stocker Road, Exeter, EX4 4QD, UK.


  Abstract

Catheter-associated urinary tract infections (CAUTI) have frequently been studied in monocultures in vitro. However, many CAUTI are due to the presence of a mixed microbial community and not just a single population, especially within patients subjected to long-term catheterization. This can potentially have important clinical implications in regards to treatment strategy and outcome. This study revealed that when Pseudomonas aeruginosa and methicillin-resistant Staphylococcus aureus (MRSA) (both commonly found in CAUTI) were grown together as a mixed culture within a CAUTI model in comparison to their respective monocultures, accelerated biofilm development was observed. Virulence gene expression analysis within P. aeruginosa and MRSA monoculture and mixed biofilms was performed through use of real-time quantitative PCR. It was revealed that production of P. aeruginosa exotoxin A was increased 1839-fold when P. aeruginosa and MRSA were grown together as a mixed biofilm. Significant expression of {alpha}-haemolysin by MRSA was not observed in either culture. It is proposed that both the biofilm-forming capability and virulence gene expression of microbes within CAUTI mixed biofilms may differ substantially to the respective microbial monocultures. This highlights the importance of developing specific treatment strategies for CAUTI when polymicrobial communities are present.

Key words: Pseudomonas aeruginosa, MRSA, urinary tract infection, Biofilm, exotoxin A, {alpha}-haemolysin

Submitted on 28 September 2007; accepted on 21 December 2007


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.